Wide-field Time-lapse Olympus IX-81 Cell-R imaging system

A Live Cell Imaging System, composed of a wide field, inverted, fully motorized Olympus IX-81 microscope equipped with:

• Olympus IX8S1F-ZDC stage (inverted) for DIC and phase contrast imaging.
• a Hamamatsu  CCD ORCA/AG cryocamera
• a fast excitation filter wheel
• Temperature and CO₂chamber
• hardware for FRET
• the CellR imaging software
• air conditioning unit

which allows fast multicolor 3D imaging, transmission microscopy (phase-contrast and Nomarski-DIC), in vivo time-lapse experiments (4D imaging), spectral unmixing of fluorescent dyes, FRET and 3D-dencovolution analysis (with the following software: No neighbor, Nearest neighbor, Inverse filter and 3D blind). The system is also equipped with video editing software (AVS video remaker, AVS video converter).